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Abstract

Assessment of ADVIA CentaurĀ® Analyzer for the Measurement of 25-OH Vitamin D by Fernando Jesus Hermida, Maria Fernandez, Belen Laborda, Aida Perez, Maria Jesus Lorenzo, Concepcion Magadan

Background: The aim of the present study was to evaluate some analytical performances of the ADVIA Centaur® analyzer for the quantitative measurement of 25-OH Vitamin D [25(OH)D] in serum.
Methods: Serum concentrations of 25(OH)D were determined by a new automated chemiluminescence immunoassay method introduced by Siemens and adapted to an ADVIA Centaur® analyzer, and compared with HPLC and a commercial chemiluminescence immunoassay (Liaison® DiaSorin).
Results: The assay displayed a low intra-day (CV < 7.0%) and a low inter-day imprecision (CV < 9.0%). The ADVIA Centaur® demonstrated a stronger Spearman´s correlation (r = 0.921), better agreement (bias = -0.3 ng/mL), and better concordance correlation coefficient of Lin (pc = 0.88) and better kappa index (k = 0.92) with HPLC, than the Liaison® DiaSorin assay (r = 0.907, bias = 5.9 ng/mL, pc = 0.84, k = 0.60) with HPLC. On the other hand, a significant inverse relationship was observed between parathyroid hormone (PTH) and 25(OH)D concentrations.
Conclusions: The ADVIA Centaur® method is reliable for routine 25(OH)D determination in clinical laboratories.

DOI: 10.7754/Clin.Lab.2012.111115