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Background: A colorimetric method based on acid violet pigment, namely AV17, to analyse salivary total protein content, was assessed.
Methods: Human saliva sample or standard (50 µL) was added to 1.5 mL of AV17 working solution (1 mg/mL in 75 mmol/L sodium chloride and 1.7 mol/L phosphoric acid). Total protein concentration was measured at 546 nm. Salivary total protein of healthy subjects was analyzed.
Results: The standard protein was Human Serum Albumin and the detection range was 38 mg/L - 900 mg/L with a LOD and LOQ of 26 mg/L and 64 mg/L, respectively. Intraday CVs were 3% - 5% and interday CVs were 3% - 6%. The dilution test demonstrated a correlation coefficient of 0.999 and the recovery tests ranged from 108% to 111%. Saliva sample stability was also demonstrated. No intra-individual salivary total protein variation was found during the morning.
Conclusions: The method suitability for laboratory diagnostic purposes to analyse human saliva protein content and stability was demonstrated.
DOI: 10.7754/Clin.Lab.2012.111006
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