Abstract
|
Design of a Biological Method for Rapid Elimination of PCR Inhibitors in Aged Bone DNA
by Akram Ghasemi, Nejat Mahdieh, Mahmood Tavallaei, Mohammad Mehdi Aslani, Zahra Zafari, Atefeh Shirkavand, Maryam Sharafi Farzad, Mahdi Naderi, Sajjad Habibi Azarban, Sirous Zeinali
|
|
|
Background: Molecular human identification is one of the most important tests performed in forensic laboratories. Some of these tests are applied for identification of human remains from natural disasters, wars, etc., but problems may occur as a result of DNA degradation and external DNA contamination. We investigated effects of bacterial DNA on identifying the presence or absence of PCR inhibitors in aged bone DNA.
Methods: DNA samples were extracted from blood, bone remains and Escherichia coli. These DNA were amplified using human and bacterial specific primers.
Results: Using different blood, aged bone, and bacterial DNA dilutions along with PCR based methods; we checked their positive, negative effects, or detecting presence of inhibitors in aged bone DNA by PCR method.
Conclusions: Our observation indicated that the addition of bacterial DNA could be a valid biological method for testing the quality of bone DNA to enable us to obtain a usable profile for the identification of human remains. This method will help to test the presence of inhibitors, quantity or even quality of DNA which are of importance in profiling archeological remains. Our method will help to determine if PCR failure is due to presence of inhibitors or lack of amplifiable DNA either because of degradation, minute amount or absence of human DNA.
DOI: 10.7754/Clin.Lab.2011.101116
|
