Abstract
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Accuracy of GFR Predictive Equations Using ID-MS Traceable Creatinine: Compensated Jaffe and Enzymatic
by Anne-Sophie Bargnoux, Noémie Simon, Valérie Garrigue, Anne-Marie Dupuy, Stéphanie Badiou, Georges Mourad, Jean-Paul Cristol
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Background: New ID-MS traceable creatinine kits including enzymatic creatinine (e-creat) and compensated Jaffe (j-creat) has been developed by Olympus.
Methods: Analytical performances of these reagents were tested on an AU®640 analyzer. A comparison study was performed against the method used in our laboratory (Randox® creatinine enzymatic). Values of the glomerular filtration rate estimated (eGFR) from ID-MS traceable equation were compared to the GFR measured (mGFR) by a reference isotope method in a kidney transplant cohort.
Results: Total intra- and inter-assay variation coefficients were below 4 %. Creatinine values were correlated with the Randox® enzymatic method (e-creat = 0.98 Randox - 0.48 and j-creat = 0.99 Randox - 1.44, r² = 0.98). Values of eGFR obtained with both e-creat and j-creat were well correlated to mGFR in our population of renal transplants.
Conclusions: The new ID-MS traceable methods improved estimation of GFR by predictive equations. However, the limitation of the Jaffe method in specific populations such as paediatric and elderly patients needs further investigations.
DOI: Clin. Lab. 2011;57:357-361
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