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Abstract |
Reverse Hybridization Assay for Rapid Identification of Periodontitis-Associated Interleukin-1 Alleles |
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Periodontitis is a chronic inflammatory disease of the periodontium characterized by an interaction between specific bacteria and the host inflammatory response. Advanced periodontal disease usually results in alveolar bone loss and loss of masticatory function. Although periodontopathic bacteria are essential for the initiation of periodontitis, the quality and quantity of plaque formation is not suffïcient to explain the different progression in disease severity. The activated host defense cells produce and release immunopathologic mediators such as cytokines or proteases. Interleukin-1 (IL-1) is one of these cytokines which is of special interest in the context of periodontitis due to its osteoclast activity properties leading to alveolar bone resorption. In the cluster of IL-1 genes there are several polymorphisms that influence the biological effects and functionality of the mature protein. ln 1997, Kornman et al. (19) identified two of these polymorphisms, which are significantly associated with severe periodontitis when found in the composite genotype comprising allele 2 of the IL-1A -889 polymorphism plus allele 2 of the IL-1B +3953 polymorphism. The genetic marker is a prognostic marker for susceptibility and predisposition to severe periodontitis. The aim of this project was to develop a reverse hybridization assay to detect the respective IL-1-polymorphisms in order to identify individuals who are highly susceptible to periodontitis. The genotyping test may provide important information and data to dentists and clinicians for treatment planning and will improve the clinicians ability to assess prognosis and predict tooth survival. |
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