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Abstract |
GC Analysis of Steroids, Fatty Acids, Organic Acids and Catecholamine Metabolites with Microwave Accelerated Derivatization for the Diagnosis of Metabolic Disorders |
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The application of microwave irradiation is described for rapid derivatization of steroids, fatty acids, organic acids and catecholamine metabolites for the diagnosis of metabolic disorders. Microwave accelerated derivatization is rapid and complete, and the products formed under microwave irradiation are identical with the products formed under conventional reaction conditions, as evidenced by similar gas chromatographic retention times. The one-step methanolysis of lipids from plasma and erythrocytes with HCI/CH3OH (1M) using a microwave oven does not result in signifïcant degradation of polyunsaturated fatty acids, even in the presence of oxygen. Capillary gas chromatographic profiles of steroids, organic acids and fatty acids for the clinical diagnosis of several diseases are discussed. |
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