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Abstract |
Relative and Absolute CDT Concentrations in Sera from Anemic Patients Measured by Commercial Immunoassays Following Miniaturized Column Chromatography and by High Performance Liquid Chromatography |
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Determination of carbohydrate-deficient transferrin (CDT) is frequently used as a laboratory tool for the diagnosis and follow-up of chronically increased alcohol consumption. To study the effect of iron deficiency anemia on CDT concentrations, 200 sera from patients presenting with decreased hemoglobin and ferritin levels were analyzed for relative CDT concentration using the new Tina-quant %CDT immunoturbidimetric assay on a Hitachi 717 analyzer (Roche Diagnostics). The results were compared to absolute CDT concentrations obtained with an established CDT radioimmunoassay (CDTect, Pharmacia & Upjohn) and to relative CDT concentrations determined by high performance liquid chromatography (HPLC) in the same sera. The data measured with the three methods show only a weak correlation (correlation coefficients < 0.5), apparently not only due to a difference between absolute and relative CDT concentrations but also to the fact that different patterns of hyposialylated isotransferrins are detected by the various assays. No sample exhibited increased CDT concentration when assayed by HPLC indicating that iron deficiency anemia does not cause marked changes in transferrin glycan composition. When the absolute CDT concentration was measured by the CDTect RIA it was slightly to markedly increased in 54 of the 200 sera tested. The Tina-quant %CDT assay, however, yielded relative CDT concentrations above the decision limit for chronically increased alcohol consumption in only 5 sera. |
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