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Background: The aim of this study is to examine the effectiveness of oscillation-induced depolymerization in mitigating pseudothrombocytopenia (PTCP) under varying oscillatory conditions.
Methods: A total of 161 patients diagnosed with PTCP and admitted between May 2020 and November 2023 were included in the study. The patients were categorized into four groups based on oscillation parameters: 1,500 rpm for 1 minute, 1,500 rpm for 3 minutes, 3,000 rpm for 1 minute, and 3,000 rpm for 3 minutes. Platelet (PLT) depo-lymerization was assessed pre- and post-oscillation in each group, and peripheral blood smears were examined to evaluate platelet distribution. Additionally, the Mindray BC-6800 Plus hematology analyzer, in combination with a special stain, was utilized to verify depolymerization and identify the most appropriate depolymerization method for clinical application.
Results: PLT counts were significantly higher in the 3,000 rpm for 3 minutes and the 3,000 rpm for 1 minute groups compared to the 1,500 rpm for 1 minute group (p < 0.05). However, no significant differences in white blood cell and red blood cell counts were observed across the oscillation conditions (p > 0.05). Depolymerization rates in the 3,000 rpm groups were significantly higher than those in the 1,500 rpm groups (p < 0.05). The oscillation-induced depolymerization rate in the 3,000 rpm for 3 minutes group reached 93.79%, which was significantly greater than that in the special stain group (11.80%) (p < 0.05). Logistic regression analysis identified elevated lymphocyte count and potassium level as risk factors for incomplete depolymerization, while total bilirubin, direct bilirubin, and indirect bilirubin levels were found to be protective factors (p < 0.05).
Conclusions: Oscillation at 3,000 rpm for 3 minutes resulted in the highest rate of platelet depolymerization and demonstrated favorable clinical efficacy for PTCP management. Monitoring of lymphocyte count, potassium, total bilirubin, direct bilirubin, and indirect bilirubin levels is essential to facilitate timely implementation of the oscillation method, thereby reducing the incidence of PTCP and enhancing the accuracy of clinical detection.
DOI: 10.7754/Clin.Lab.2025.250420
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