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Effect of 6-Hour Exposure to 20°C on the ATP Content and other Biochemical Measures of CPDA-1 Packed Red Cells by Thomas Ecker, Walter E. Hitzler

Blood donations for clinical use are routinely stored, at 2°C to 6°C for 35 to 42 days. It is common practice for RBCs exposed to temperatures above 10°C to be destroyed, although the American Association of Blood Banks Technical Manual states "Blood exposed to temperatures above 10°C is not necessarily unsuitabte for transfusion". To clarify this issue we investigated the effect of 6-hour storage at 20°C on the content of ATP and other biochemical measures of CPDA-1 packed red cells. CPDA-1 packed RBC units were exposed once at day 5 (group 1), day 15 (group 2) or day 30 (group 3) of their shelf life to 20°C for 6 hours. Control groups were continuously refrigerated. Under all conditions of storage, the ATP concentrations decreased with time. Initial ATP tevels of five-day old CPDA-1 packed RBCs were 3.94 µmol/g Hb in the test group and 3.73 µmol/g Hb in the control group. At day 30 after warming (day 35 of the shelf-life) the ATP concentrations declined to 2.78 µmol/g Hb (test group) and to 3.55 µmol/g Hb (controls). In the test series which were warmed at day 15 and day 30 of shelf-life the ATP levels declined to 3.16 µmol ATP/g Hb and 2.92 µmol ATP/g Hb at day 35 of shelf-life. There was no signifïcant difference between test and control group with respect to the lactate levels, whole-blood glucose, sodium and potassium. The percentage of hemolysis was lower than 0.5% under all conditions of storage. Our data show that a shorter period of moderate warming (6 hours, 20°C) does not lead to a critical decline of ATP and glucose concentrations in CPDA-1 packed RBCs. The survival of RBCs stored in CPDA-1 is most highly correlated with maintaining ATP concentrations above a value of about 2 µmol per g of Hb [3]. The ATP levels in our study were well above this threshold.

DOI: Clin. Lab. 2000; 46:291-293