Abstract
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3D Culture System for Human Adrenal Glands That Uses a Sequential Processing Medium to Facilitate Cortical-Medullary Cell Development
by Yajie Guo, Bingqian Guo, Yan Huang, Yu Ye, Hongfei Chen, Liying Zhang, Minxi Li, Yifu Wang, Yufang Lv, Jinling Liao, Yang Chen, Yu Long, Jinghang Jiang, Zhongyuan Chen, Yi Guo, Zengnan Mo, Yonghua Jiang
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Background: The human adrenal gland is composed of the cortex and the medulla, which contain different function cells. The aim of this study was to build a 3D culture system for human adrenal glands.
Methods: Human fetal adrenal tissues were digested into a cell suspension culture and processed in three-phase 3D cultures.
Results: Apparent spheroids could be seen from the 4th day on. After 21 days of 3D culture, steroid synthesis cells were evident via CYP17A1+ immunohistochemical staining and flow cytometry analysis. Electron microscopy analysis showed that these cells were present in lipid droplets in the cytoplasm. Meanwhile, TH+ cells represented catecholamine-producing cells, and these cells exhibited electron density particle gathering in the cytoplasm. Dehydroepiandrosterone and epinephrine syntheses were further confirmed via enzyme-linked immunosorbent assay.
Conclusions: We established a 3D culture system for human adrenal glands by using a sequential processing medium to facilitate cortical–medullary cell development.
DOI: 10.7754/Clin.Lab.2024.240719
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