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Consistency Analysis of the UF-1500 and UF-5000 Automated Urine Particle Analyzers by Masato Hoshi, Kazuko Nagashima, Masayo Sakurai, Yuka Inoue, Misaki Terashima, Takashi Fujita, Hiroyasu Ito

Background: The aim was to evaluate the consistency of the results between the UF‐1500 and UF-5000, fully automated urine particle analyzers.
Methods: A total of 554 randomly selected inpatient and outpatient urine samples were collected for analysis using the UF‐1500, the UF-5000, and by manual microscopic examination. The coincidence rate, intraday repeatability, and interday reproducibility were evaluated on the UF‐1500 and UF-5000. To analyze the review flags from the UF-1500, the UF-1500 results were compared to manual microscopy as the gold standard.
Results: The repeatability of red blood cells (RBCs), white blood cells (WBCs), epithelial cells (ECs), casts, and bacteria using the UF-1500 and UF-5000 is expressed as the relative standard deviations of the intraday and inter-day measurements. For the UF-1500, the relative standard deviation values ranged from 5.9% to 12.6% and 4.9% to 17.2% for the low and 1.6% to 9.3% and 2.3% to 16.9% for the high samples, respectively. The correlation co-efficient for RBCs, WBCs, ECs, SECs, casts, crystals, and bacteria for the UF-1500 were 0.981, 0.993, 0.968, 0.963, 0.821, 0.783, and 0.992, respectively. Review samples from the UF-1500 were confirmed by microscopic examination. Review flags for all 554 samples included 3 samples with “DEBRIS High” and 23 samples with “RBCs/YLC Abnormal classification”.
Conclusions: The identification of various urine components by both instruments meets laboratory requirements. These two instruments with different performances have specific characteristics and should be used based upon the needs of each laboratory.

DOI: 10.7754/Clin.Lab.2023.230940