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Abstract |
B-type Natriuretic Peptide (BNP) - Validation of an Immediate Response Assay |
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B-type natriuretic peptide, a proteohormone secreted by the left ventricle in response to wall-tension, is a promising laboratory parameter for the detection and follow-up of heart failure. In this report analytical validation data of a non-isotopic point-of-care testing system for the quantitative determination of BNP (Triage BNP, Biosite, USA) are given. Despite a very short turn-around time of about 10 minutes the assay proved to be reproducible (interassay coefficient of variation (n = 10) of 8.4% and 8.0% at concentrations of 19.3 ng/L and 392 ng/L, respectively), linear (r = 0.998, from 5 ng/L to 818 ng/L), and rugged with respect to common interferents; compared to the widely used SHIONORIA BNP assay (CIS, France) higher results were found (Triage-BNP = 1.52 x SHIONORIA BNP - 7,0 ng/L; n = 70) with a relatively close correlation of the results (r = 0.935). It is concluded that the Triage BNP assay meets the analytical requirements for further clinical validation and may allow a more widespread clinical use of BNP determination in contrast to competing assays with long turn-around times. |
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