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Glycosylated Bence Jones Protein with Poor Thermal Reactivity in Heat Coagulation Tests by Mayumi Imoto, Katsunori Watanabe, Koji Yoshida, Ken-ichi Nakae, Toshinori Kamisako, Toshiyuki Yamada

Background: We experienced a patient with multiple myeloma whose urine contained a considerable amount of Bence Jones protein (BJP), which demonstrated poor thermal reactivity in heat coagulation test. The mechanism for this phenomenon was assessed.
Methods: Immunoelectrophoretic analyses reveal that a band corresponding to BJP in the urine had 2,600 Dalton by reduction after glycosidase treatment, but not after sialidase treatment. In addition, the glycosidase-treated urine tested positive in heat coagulation test.
Conclusions: Glycosylation of the immunoglobulin light chain, which has rarely been seen, is the cause of the unexpected behavior of this patent’s BJP in heat coagulation tests.

DOI: 10.7754/Clin.Lab.2020.200304