You have to be registered and logged in for purchasing articles.


Increasing DNA Isolation Performance from Blood Cells Using a Modified Commercial Low-Cost Reagent by Mauricio N. Kraemer, María V. Mencucci, Martín Abba, Ezequiel Lacunza, Juan J. Gagliardino

Background: Genomic studies facilitate comprehension of the pathophysiology, diagnosis, and treatment of chronic diseases. Such studies require sufficient and good quality DNA isolated from a large number of blood samples. This study attempts to obtain a high-quality genomic DNA isolated from a large number of blood samples using a simple and cheap method.
Methods: The EasyPure® Genomic DNA Kit (Transgen Biotech) was modified to increase the amount of DNA recovery: a few steps and two additional column elutions were added to the original manufacturer´s procedure.
Results: The amount of DNA isolated from frozen blood samples increased by an average of 56%. Its 260/280 ratio and electrophoretic mobility properties make it suitable for genomic studies.
Conclusions: A relatively low-cost commercial column and a simple modification of the manufacturer´s protocol, provided a simple and cheap procedure to isolate high-quality DNA from a large number of blood samples suitable for genomic studies.

DOI: 10.7754/Clin.Lab.2019.190349