Background: To explore the role of resistin (RE) on deep vein thrombosis (DVT) and the molecular mechanism.
Methods: ELISA assay was used to determine the concentration of RE, MMP-2, MMP-9, and PAI-1 in serum of DVT patients and controls. Rats were randomly divided into 4 groups: control, DVT, DVT + RE, and DVT + RE + SB203580 group. HE staining was used to observe the intravascular situation and thrombosis in the 4 groups. The relative mRNA levels and protein expression of RE, MMP-2, MMP-9, and PAI-1 in the 4 groups were determined by RT-PCR and western blotting.
Results: The concentration of RE, MMP-2, MMP-9, and PAI-1 in serum from DVT patients was significantly higher than that in controls. RE promoted thrombosis in rats with DVT, while SB203580 inhibited the promotion of RE on thrombosis. RE up-regulated the expression of MMP-2, MMP-9, and PAI-1 in DVT rat models. Introduction of SB203580 inhibited the expression of RE, which then abolished the up-regulation role of RE on the expression of MMP-2, MMP-9, and PAI-1.
Conclusions: RE promoted thrombosis in DVT rat models by increasing the expression of MMP-2, MMP-9, and PAI-1.