Background: The current study aims to investigate the expression of miR-1 in serum of patients with Kawasaki disease (KD) and its clinical significance.
Methods: The serum samples of 33 patients with KD and 15 healthy people were collected from January 2017 to June 2017 at the Affiliated Hospital of North China University of Science and Technology. The expression of serum miR-1 was detected by real-time quantitative PCR (RT-qPCR). The diagnostic value of miR-1 as a marker of KD disease was evaluated by receiver operating characteristic (ROC) curve.
Results: The level of miR-1 in serum of children with acute KD was significantly higher than that of healthy children, but it decreased to normal level in convalescence. ROC curves showed that the area under the curve (AUC) of miR-1 for KD diagnosis was 0.754 (95% CI: 0.541 - 0.952). When the critical value (diagnostic threshold) was 1.08, the diagnostic sensitivity and specificity were 0.867 and 0.735, respectively. In addition, we further divided the KD patients according to clinical characteristics. Here, we showed that the expression of miR-1 was higher in the serum of KD patients with higher platelet level.
Conclusions: In summary, serum miR-1 in patients with acute KD was significantly increased, which may be one of the potential serum markers for the diagnosis of KD.