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Objective: To explore the relationship between the RANTES, TGFβ1 and amount of mast cells (MC) surrounding the implanted tumors.
Method: Pieces of Walker 256 carcinosarcoma were implanted in the liver of 40 male Wistar rats and the formed intrahepatic implanted tumors were then divided into 3 groups: group without MC infiltration, group with little MC infiltration and group with MC infiltration; 8 normal rats served as control group. The sera of rats in the different groups were tested by ELISA to find the serum RANTES content of the tumor bearing rats, then the chemotactic activity of the serum RANTES of different tumor bearing groups vs peritoneal MC of normal rats was tested by the microBoyden chamber.
Results: The MC amount of the tumor bearing rats was quite different, some of them showed a significantly increased amount. The groups with more MC infiltration showed a higher RANTES content in the sera and a stronger chemotactic activity vs MC.
Conclusion: The RANTES was an effective chemotactic factor to MC. The serum concentration of RANTES of the tumor bearing rats is related to the difference of the MC amount surrounding the tumor.
DOI: Clin. Lab. 2003;49:65-69
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