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Background: Two single-nucleotide polymorphisms (SNPs) rs12979860 (C/T) and rs8099917 (T/G), near the interleukin 28B (IL28B, also known as IFN lambda-3, IFNL3) gene, have a strong association with both spontaneous and treatment-induced hepatitis C virus (HCV) clearance. IL28B polymorphisms have shown a marked differential distribution with regional and ethnic variation. The study aimed to investigate the distribution of IL28B rs12979860 and rs8099917 genotypes in patients with chronic hepatitis C (CHC) in Tianjin, China.
Methods: A total of 1,600 patients with chronic HCV infection were enrolled, including 762 males and 838 females. The IL28B rs12979860 and rs8099917 polymorphisms were genotyped via a DNA microarray-based assay.
Results: The distribution of IL28B rs12979860 CC, CT, and TT genotypes was 93.94%, 5.94%, and 0.12%, and that of rs8099917 TT, TG, and GG genotypes was 88.75%, 11.06%, and 0.19%, respectively. The allele frequencies for rs12979860 C and T were 96.91% and 3.09%, and those for rs8099917 T and G were 94.28% and 5.72%, respectively. The combined assessment of the two SNPs showed that rs12979860 CC/rs8099917 TT was more prevalent with a frequency of 85.94% (1375/1600). When stratified with gender, the genotype and allele distribution of the two SNPs did not significantly differ between male and female subjects. When compared with the data derived from previously reported studies and the 1000 Genomes Project, there was a relatively higher distribution of favorable genotypes rs12979860 CC and rs8099917 TT in the present study.
Conclusions: IL28B rs12979860 CC and rs8099917 TT genotypes were predominant in patients with chronic HCV infection in Tianjin, China. These data provided new insight into the geographical frequency distribution of IL28B variants. The higher distribution of the favorable genotypes in Tianjin might be suggestive of better response to antiviral therapy for interferon-eligible CHC patients in this resource-constrained area.
DOI: 10.7754/Clin.Lab.2017.171036
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