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Abstract |
A New Olympus® Assay for the Determination of Ceruloplasmin |
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Ceruloplasmin’s main clinical importance is in the diagnosis of Wilson’s disease, where plasma ceruloplasmin concentration is reduced. Increased levels are particularly notable in diseases of the reticuloendothelial system such as Hodgkin’s disease as well as during pregnancy or use of contraceptive pills. This paper describes a new Olympus® assay OSR6164 for the determination of ceruloplasmin on the Olympus® AU640E, AU400E and AU2700 analytical systems. The turbidimetric assay has a linear measurable range of 60-2000 mg/l. Prozone hook effect greater than 9000mg/l. Imprecision CV values <5% for within-run and <10% for between-day. Lipemic, hemolytic and icteric interference of ≤5%. Traceability of recovery to CRM470 standardisation. Method comparison to Behring Nephelometry yielded a correlation (r) of 0.99 and a slope of 1.02. On-board and calibration stability of 60 days and 14 days, respectively. Serum or plasma (EDTA and lithium heparin anticoagulants) samples may be used. |
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