Abstract
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Detecting of p16 Autoantibody as a Potential Early Diagnostic Serum Biomarker in Patients with Cervical Cancer
by Mingmei Huangfu, Linlin Liu, Shuang Xu, Siyao Li, Kuo Jiang, Baosheng Sun, Kuang-Hui Lee, Shilong Sun
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Background: Over-expression of tumor-associated antigens (TAAs) may trigger secretion of their auto-antibodies. The present work was designed to test whether circulating antibody to P16 protein-derived antigens was altered in cervical cancer.
Methods: 141 cases of cervical cancer patients, 133 cases of cervical benign tumor patients, and 153 healthy volunteers matched in age were recruited. The level of circulating P16 auto-antibody was tested using an ELISA developed in-house with linear peptide antigens derived from the P16 protein.
Results: The P16 auto-antibody in the malignant tumor group had a significantly higher level than the healthy control group and the benign tumor group (t = 4.016, p < 0.001; t = 3.879, p < 0.001). Patients with stage I cervical cancer have the highest level of P16 autoantibody and the sensitivity against > 90% specificity was 20.3%.
Conclusions: The circulating auto-antibody to P16 may be one of a series of potential biomarkers with early prognostic values for cervical cancer.
DOI: 10.7754/Clin.Lab.2015.151024
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