Abstract
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High-Sensitivity Cardiac Troponin T: a Preanalytical Evaluation
by Moise Michel, Fouzi Mestari, Rana Alkouri, Gregory Atlan, Sylvie Dever, Catherine Devilliers, Francoise Imbert-Bismut, Dominique Bonnefont-Rousselot, Denis Monneret
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Background: Little is known about the effects of preanalytical conditions on high-sensitivity cardiac troponin T (hs-cTnT) concentrations.
Methods: Variations of hs-cTnT concentrations were evaluated under the following preanalytical conditions: 1) serum vs. lithium-heparin (Li-Hep) plasma, with or without separator gel; 2) centrifugation time (15-minutes vs. 10-minutes) and speed (1467 to 3756 g); 3) stability in Li-Hep plasma at room temperature and +4°C for 4 days and at -80°C for up to 12 months, for three concentrations; 4) four freeze-thaw cycles at -20°C and -80°C, for three concentrations.
Results: No significant changes were found regarding the type of blood collection tube, the centrifugation, and storage conditions. Minor decreases were observed after four freeze-thaw cycles at -20°C (< 6.5%) and -80°C (< 3.4%).
Conclusions: High-sensitivity cardiac troponin T may be considered as not impacted by usual preanalytical conditions, thus strengthening its reliability in laboratory practice and clinical research.
DOI: 10.7754/Clin.Lab.2016.160123
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