Abstract
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STC1 and NF-κB p65 (Rel A) is Constitutively Activated in Colorectal Cancer
by Saleheh Rezapour, Tayyeb Bahrami, Shahryar Hashemzadeh, Mehrdad Asghari Estiar, Masoumeh Nemati, Reyhaneh Ravanbakhsh, Mohammad Ali Hosseinpour Feizi, Hossein Samadi Kafil, Nasser Pouladi, Morteza Ghojazadeh, Ebrahim Sakhinia
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Background: Stanniocalcin-1 (STC1) and nuclear factor (NF)-ƙB subunit p65 transcription factor are involved in various types of human malignancies. The roles of STC1 and NFƙB-p65 in colorectal cancer (CRC) are still not fully understood. We investigated expression levels of NF-ƙB p65 and STC1 and also correlations between STC1 and NF-ƙB p65 expression and clinicopathological features in CRC.
Methods: Tumor tissue samples were collected from 48 patients with CRC. RT-PCR and Real-time PCR analysis was performed to examine mRNA levels of STC1 and NF-ƙB p65.
Results: The relative mRNA levels of STC1 and NF-ƙB p65 were significantly higher in tumor tissues than in adjacent mucosa (p = 0.025 and p = 0.044, respectively). The data also showed that STC1 and NF-ƙB p65 mRNA levels were not significantly associated with clinicopathological characteristics. In addition, there was no association between expression levels of STC1 and NF-ƙB p65 in tumor samples.
Conclusions: Our data indicate that STC1 and NF-κBp65 is activated constitutively in colorectal carcinoma tissues, suggesting that activation of these factors might play an important role in colorectal tumorigenesis. Future studies should examine STC1 and NF-κBp65 as a molecular target for the treatment of CRC.
DOI: 10.7754/Clin.Lab.2015.150827
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