Abstract
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A Single Nucleotide Polymorphism in the FOXP3 Gene Associated with Behçet’s Disease in an Iranian Population
by Arezoo Hosseini, Dariush Shanehbandi, Mehrdad Asghari Estiar, Saber Gholizadeh, Alireza Khabbazi, Hamidreza Khodadadi, Ebrahim Sakhinia, Zohreh Babaloo
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Background: Behçet’s Disease (BD) is a rare autoimmune disease that involves the dysfunction of regulatory T cells. FOXP3 is a key transcription factor in the development and function of Treg cells. Recent studies have shown SNPs in the FOXP3 contribute to the susceptibility to some autoimmune disorders.
Methods: To clarify the association between the FOXP3 gene and the risk of BD, 50 patients diagnosed with BD and 50 healthy controls from north-western Iran were genotyped by PCR-RFLP (Mun I and Pst I) for two SNPs including rs3761547 (-3499T/C) and rs3761548 (-3279 C/A) in the promoter region of the FOXP3 gene. In addition, a 506 bp nucleotide sequence of FOXP3 promoter was analyzed.
Results: The allele -3279 C/A was significantly associated with BD [p = 0.002; odds ratio (OR) = 3.841; 95% confidence interval (CI) 1.610 - 9.161]; whereas, there was no contribution of the FOXP3 polymorphism -3499T/C to BD [(p = 0.084); (OR = 0.348, 95% CI = 0.101 - 1.195)]. Meanwhile, sequence analysis showed 100% similarity in both controls and BD patient groups.
Conclusions: Therefore, the SNP rs3761548 in the FOXP3 gene appears to contribute to the risk of Behçet’s disease among the north-western Iranian population.
DOI: 10.7754/Clin.Lab.2015.150433
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