Abstract
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Feasible Quantitative Detection of Cytomegalovirus from Urine Sediment in Stem Cell Transplant Patients
by Min-Gu Kang, Min-Joong Jang, Seung-Yeob Lee, Hye-Ran Kim, Seok-Yong Choi, Jong-Hee Shin, Soon-Pal Suh, Dong-Wook Ryang, Myung-Geun Shin
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Background: Urine is an important source for the detection of infections caused by CMV in stem cell transplant patients. Currently, there is no agreement about the type of urine specimen. In order to investigate which is the better specimen type for quantitative detection of CMV, we compared the results from urine supernatant and sediment from the same patients.
Methods: Seventy urine specimens were collected from patients with hematological disorders or solid tumors. After performing shell vial culture, residual urine specimens were centrifuged. Then, 10 mL of each urine supernatant and sediment were taken and immediately frozen at -70°C. Afterwards, archived urine specimens were thawed at room temperature and CMV-quantitative PCR was performed on both the supernatant and sediment fraction of urine. The results from each patient were reviewed for CMV antigenemia, blood shell vial culture, CMVIgM or IgG, and clinical symptoms.
Results: CMV-qPCR results for the urine sediment fraction revealed a significant difference (p = 0.012) between the active CMV infection group and the latent CMV infection group. In addition, receiver operating characteristic curves for active CMV infection revealed that CMV-qPCR using urine sediment produced more accurate results than urine supernatant.
Conclusions: These findings suggest that the sediment fraction of urine is a more suitable specimen in CMV-qPCR testing.
DOI: 10.7754/Clin.Lab.2014.140810
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