You have to be registered and logged in for purchasing articles.

Abstract

Assessment of an Expert System for the Automated Validation of Electrophoretic Profiles by Romolo M. Dorizzi, Valerio Zanardi, Riziero Agnoletti, Anna Alberelli, Alessandra Babini, Paolo De Vita

Background: The Core-lab of the Greater Romagna Area Hub Laboratory carries out about 250,000 capillary electrophoresis assays/year. The huge workload demands the assessing of an Experimental Expert System (EES) capable to sort out the negative samples.
Methods: Capillarys 2 analyzer has been employed coupled with an EES (based on five simple rules) integrated with the electrophoretic test management software PhoresisCore for assessing the entire workload of a week (5,683 samples). The classification was compared with that of two expert laboratorians.
Results: The expert system automatically classified 2974 profiles as negative and no positive samples were erroneously classified as negative (negative predictive value: 100%).
Conclusions: The EES sensitivity was 100% and the FTE required for the validation was reduced from 1.26 to 0.63. The EES could be easily implemented in routine activity embedded in a middleware or directly running in the analyzer improving the workflow.

DOI: 10.7754/Clin.Lab.2014.140715