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Expression of Subtypes of Interleukin-17 Ligands and Receptors in Patients with B-Cell Chronic Lymphocytic Leukemia by Marzena Garley, Ewa Jablonska, Jolanta Sawicka-Powierza, Wioletta Ratajczak-Wrona, Janusz Kloczko, Jaroslaw Piszcz

Background: The diversity of biological effects of cytokines from the IL-17 family, as well as the wide range of cells sensitive to their influence, suggests that these molecules might play a significant role in the malignant process.
Methods: After the cells were initially isolated with Polymorphprep™, they were sorted with a MACS® magnetic separator, CD16 for neutrophils and CD19 for B lymphocytes. The presence of proteins: IL-17A, IL-17E, IL-17F, IL-17D, as well as receptors: IL-17R, IL-17BR, IL-17RC in cell lysates was confirmed by Western blot. The levels of IL-17A, IL-17E, and IL-17F in blood serum were determined with ELISA.
Results: The results indicate a lower expression of IL-17A, IL-17E, and IL-17F in PMNs and B lymphocytes of patients with B-cell chronic lymphocytic leukemia compared to cells of healthy subjects. Elevated expression of IL17D was observed in the PMNs of patients, with a simultaneous decrease in the expression of this cytokine in leukemic B cells, in comparison to the control group. In patients with B-CLL, there also were observations of decreased expression of IL-17R, IL-17BR, and IL-17RC in leukemic B lymphocytes, compared to their expressions in the cells of healthy subjects. The blood serum of B-CLL patients demonstrated a significantly increased level of IL-17A at stage 0/I, II, and IV of disease; IL-17E at stage 0/I and II; IL-17F at stage 0/I, III, and IV of disease advancement, compared to the data obtained from the control group.
Conclusions: The results clearly support the involvement of the studied proteins from the IL-17 family in the course of B-CLL and indicate that IL-17D may play a significant role in the course of this disease.

DOI: 10.7754/Clin.Lab.2014.131107