Abstract
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Suppression of T-Type Ca2+ Channels Inhibited Human Laryngeal Squamous Cell Carcinoma Cell Proliferation Running Title: Roles of T-Type Ca2+ Channels in LSCC Cell Proliferation
by Wenfa Yu, Ping Wang, Huiming Ma, Guozheng Zhang, Yulin Zhao, Baocai Lu, Huiming Wang, Mingmin Dong
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Background: Despite the tight correlation between T-type Ca2+ channels and a great variety of tumors, the roles of
α1G subunit of T-type Ca2+ channels in laryngeal squamous cell carcinoma (LSCC) have not yet been investigated.
Methods: In the present study, we examined the expression of α1G subunit of T-type Ca2+ channel in human
LSCC tissues and cell lines. One human laryngeal squamous cell carcinoma cell line, Hep-2, was also examined for
T-type channels using voltage-clamp recordings. Cell proliferation assays were performed in the presence or absence
of T-type channel blocker mibefradil and α1G subunit sepcific siRNA. The cell cycle was determined by
flow cytometry.
Results: Our results indicated that the α1G subunit of T-type Ca2+ channel is highly expressed in human laryngeal
squamous cell carcinoma tissues and cell lines. α1G siRNA significantly down-regulated the protein expression of
the α1G subunit. Both α1G siRNA and mibefradil inhibited Hep-2 cell proliferation and arrested cell cycle progression.
Conclusions: Together, these findings suggest a functional role of T-type channels in certain laryngeal carcinomas,
and that inhibition of T-type channels reduces cell proliferation via cell cycle arrest, suggesting that the α1G subunit
of T-type Ca2+ channel may be used as a therapeutic target for treating LSCC.
(Clin. Lab. 2014;60:621-628. DOI: 10.7754/Clin.Lab.2013.130614)
DOI: 10.7754/Clin.Lab.2013.130614
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