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Abstract |
Evaluation of Rezasurin Microtiter Assay and High Resolution Melting Curve Analysis for Detection of Rifampicin and Isoniazid Resistance of Mycobacterium tuberculosis Clinical Isolates |
Background: Several methods have been proposed for rapid detection of drug resis-tance of Mycobacterium tuberculosis (M. tuberculosis). High resolution melting HRM) curve analysis has been developed for accurate and simultaneous detection of resis-tance of M. tuberculosis isolates to rifampicin (RIF) and isoniazid (INH). Other techniques include the resazurin microtiter assay (REMA) which is one of the new colorimetric phenotypic methods. The present study evaluated the HRM curve analysis and REMA assay in comparison to the proportional method (PM) for rapid identifica-tion of multidrug resistant (MDR) M. tuberculosis isolates.
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