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Background: To compare the results of HbA1c determination obtained through immunoassays versus the HPLC method currently used routinely in our laboratory.
Methods: We evaluated immunoturbidimetric assays for the HbA1c measure on three analyzers, specifically the Roche Cobas Integra 400+® (Roche Diagnostics, Indianapolis, IN, USA), Ortho Clinical Diagnostics Vitros 5.1 FS® (Ortho Clinical Diagnostics, NY, USA), and Siemens Dimension RxL® (Siemens Healthcare Diagnostics, NY, USA), in comparison with the HPLC Menarini HA 8140® (Menarini Diagnostics, Rungis, France) currently used in our laboratory.
Results: Analytical performances including precision, analytical range, recovery, carryover, erythrosedimentation and comparison studies were acceptable leading to results with a level of exactitude in accordance with the recom-mendations of the National Glycohemoglobin Standardization Program (NGSP).
Conclusions: The three immunoassays tested can be used interchangeably and will be satisfactory for laboratories who cannot invest in a HPLC analyzer.
DOI: 10.7754/Clin.Lab.2012.111222
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