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Abstract

Comparison of Automated 25-OH Vitamin D Immunoassays with Liquid Chromatography Isotope Dilution Tandem Mass Spectrometry by Marja-Kaisa Koivula, Ursula Turpeinen, Päivi Laitinen, Juha Risteli

Background: Comparing two fully automated 25-OH-D immunoassays with a validated liquid chromatography isotope dilution tandem mass spectrometry (LC-IDMS/MS) method in human serum samples.
Methods: 180 samples were analyzed with the Liaison® TOTAL Vitamin D, the IDS-iSYS 25-Hydroxy Vitamin D, and a validated LC-IDMS/MS 25-OH D method.
Results: The Liaison® method mean ± standard deviation (SD) was 64.3 ± 23.5 nmol/L; the IDS-iSYS 70.1 ± 27.0 nmol/L, and the LC-IDMS/MS 68.9 ± 24.6 nmol/L. The correlation coefficient (r) between the Liaison® and LCIDMS/MS was 0.723, and the r between the IDS-iSYS and LC-IDMS/MS was 0.799. When the samples were grouped by females and males, the r between the Liaison® and LC-IDMS/MS was 0.680 and 0.846, being 0.781 and 0.863 between the IDS-iSYS and LC-IDMS/MS, respectively. With the Passing-Bablok analysis, only the IDSiSYS gave equivalent results to LC-IDMS/MS.
Conclusions: The automated 25-OH-D immunoassays were in clinically good overall agreement with the LCIDMS/MS method.

DOI: 10.7754/Clin.Lab.2012.111103