You have to be registered and logged in for purchasing articles.

Abstract

Rapid Detection of the R389G Polymorphism of ADRB1 by Real Time PCR Using Fluorescence Resonance Energy Transfer. by Matthias Sauer, Michael Schwarzer, Joerg Seeburger, Volker Adams, Volkmar Falk, Stefan Dhein, Friedrich-Wilhelm Mohr

The β1-adrenoceptor plays a crucial role in the regulation of myocardial contractility and heart rate. A functionally important polymorphism at codon 389 has been associated with heart failure and hypertension. The conventional method of detecting this polymorphism is a PCR-assay followed by digestion with specific restriction enzyme (PCR-RFLP) polymorphism. We aimed to establish an alternative, fast genotyping method by real time PCR, which combines high speed DNA-amplification and real time fluorescence monitoring using fluorescence resonance energy transfer. Venous blood of 50 healthy volunteers was examined for the Arg389Gly polymorphism using both methods. The results from PCR-RFLP were compared to those obtained with real time fluorescence PCR and showed a 100% correlation. DNA sequencing confirmed a 100% concordance. This alternative technique is significantly less time consuming than traditional PCR-RFLP and facilitates the analysis of a large numbers of samples.

DOI: Clin. Lab. 2009;55:128-136